Abstract
Objective: This study aims to isolate an active phytoconstituent from ethanolic leaf extract of Pothos scandens Linn., to evaluate in vitro anticancer activity, and to carry out molecular docking studies of the isolated phytoconstituent. Methods: The bioactive constituent 1,1’-(4,5-dihydroxy benzene-1,2-diyl) bisoct-7-en-1-one, a phenolic compound, was isolated by using chromatographic methods and the structure was elucidated by various spectroscopic techniques. In vitro anticancer activity was evaluated against HeLa and MCF 7 cell lines. The viability of cells was evaluated by direct observation of cells by an Inverted phase-contrast microscope and by the MTT assay method. IC50 was calculated using the linear regression model. Results: The results of anticancer studies revealed that different concentrations of the ethanolic extract of leaves of Pothos scandens Linn. exhibited cytotoxic activity against HeLa and MCF 7 cell lines with IC50 of 22.9 and 18.32 μg/ml, respectively. The anticancer potential of the plant was revalidated by in silico molecular docking study with Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, PDB ID: 4AG8) using Discovery studio 2018. Results of the docking study showed that the ligand exhibited strong interaction with the VEGFR2 kinase with significant binding energy. Conclusion: Pothos scandens linn. can be used for the isolation of potent anticancer agents.
Highlights
Cancer is a disease in which some cells of our body grow abnormally and invade other parts of the body
Anticancer agents are used for curing cancer or to prolong life or reduce symptoms associated with cancer [2]
A phytoconstituent 1,1’-(4,5-dihydroxy benzene-1,2-diyl)bisoct7-en-1-one was isolated from ethanolic leaf extract of Pothos scandens Linn and its structure was elucidated with the help of spectral data
Summary
Cancer is a disease in which some cells of our body grow abnormally and invade other parts of the body. The normal cell cycle control is disrupted by: Abnormal growth factor function, Abnormal cyclin-dependent kinase (CDK) function, Abnormal DNA synthesis, and Abnormal decreases in negative regulatory forces due to mutation in the tumour suppressor gene [1]. The plant-based therapeutics provide an alternative strategy for better and less toxic cancer treatment [4]. Secondary plant metabolites such as alkaloids, flavonoids, polyphenols, saponins, lignans, volatile oils, terpenes, etc exhibit significant anticancer effects either by inhibiting cancer cell-activating proteins, enzymes and modulating multiple signaling pathways such as CDK2, NF-κB, Bcl-2, cytokines, PI3K, Akt, MAPK/ERK, MMP, or by activating DNA repair mechanism [5,6,7]
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