Abstract
Background and Aim:Toll-like receptors (TLRs) comprise microbial sensing receptors present on cell surfaces that are capable of detecting pathogens. The present study aims to examine the expression of TLRs within the peripheral blood mononuclear cell (PBMC) of the Betong chickens.Materials and Methods:Blood samples were harvested from 12 Betong (KU line) chickens. Hematological values were calculated. PBMC was isolated from the blood utilizing a Histopaque solution and stored in a RPMI1640 culture medium. Cell viability was investigated using a Trypan Blue dye exclusion test. DNA was extracted from PBMC and the expression of the DNA’s TLRs was examined using a polymerase chain reaction.Results:Hematological values were determined from the blood samples collected in this study obtained from healthy Betong chickens. PBMC that was isolated from the Betong chickens possessed cell viability higher than 95% (95.37±1.06). From the examination of TLRs gene expression, results revealed instances of TLR1.1, TLR1.2, TLR2.1, TLR2.2, TLR3, TLR4, TLR5, TLR 7, TLR15, and TLR21 that were present in the PBMC of Betong chickens.Conclusion:PBMC isolated from the blood of healthy Betong chickens possessed excellent cell quality. All chicken TLRs were discovered within the PBMC of Betong chickens. Hence, PBMC stands out as one of the premier sources for in vitro studies of chicken immune response.
Highlights
Toll-like receptors (TLRs) belong to the pattern recognition receptor system, which detects pathogens through their recognizable molecular patterns, which include multiple components of pathogens including lipopolysaccharides, peptidoglycans, flagellin, bacterial DNA, and viral double-stranded RNA [1]
peripheral blood mononuclear cell (PBMC) that was isolated from the Betong chickens possessed cell viability higher than 95% (95.37±1.06)
From the examination of TLRs gene expression, results revealed instances of TLR1.1, TLR1.2, TLR2.1, TLR2.2, TLR3, TLR4, TLR5, TLR 7, TLR15, and TLR21 that were present in the PBMC of Betong chickens
Summary
Toll-like receptors (TLRs) belong to the pattern recognition receptor system, which detects pathogens through their recognizable molecular patterns, which include multiple components of pathogens including lipopolysaccharides, peptidoglycans, flagellin, bacterial DNA, and viral double-stranded RNA [1]. TLRs orchestrate a vital role within the innate immune response, and TLRs are expressed within a wide scope of tissues and cell types [2]. TLRs characteristics were detected in several types of chicken tissues. Peripheral blood mononuclear cells (PBMCs) consist of a mixed population of single nuclear white. Toll-like receptors (TLRs) comprise microbial sensing receptors present on cell surfaces that are capable of detecting pathogens. The present study aims to examine the expression of TLRs within the peripheral blood mononuclear cell (PBMC) of the Betong chickens
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