Isolation of peat swamp forest foliar endophyte fungi as biofertilizer

Safinah Surya Hakim,Sari Nurulita,Tri Wira Yuwati

doi:10.20527/jwem.v5i1.111

Safinah Surya Hakim, Sari Nurulita + Show 1 more

Open Access

https://doi.org/10.20527/jwem.v5i1.111

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Abstract

Peatland restoration activity is facing many obstacles, particularly in planting techniques and poor nutrient in peat soil. Naturally, endophytic fungi are abundant and have great potential as biofertilizer. This research investigates the potential endophytic fungi isolated from leaves of peat swamp tree species for biofertilizer. Research activities include: exploration, in vitro test to examine the phosphate solubilization and identification. Result showed that there were 360 leave segments collected from 4 sampling locations. The colonization percentage of 222 isolates ranged from 52.17% - 60.17%. Fifty seven morphospecies were selected from 222 isolates. Twelve isolates demonstrated ability to produce clear zones and ten isolates were selected for identification. It is concluded that twelve isolated demonstrated potential ability to produce clear zone and Penicillum citrinum isolate P3.10 was identified as an isolate that show the highest potential ability as a biofertilizer

Highlights

Fungi are abundant in our environment, and it’s recorded that there were almost 1.5 million of species fungus were found on the earth (Hawksworth, 2004)
Endophytic fungi is fungi which live within plant tissues, including in leaves tissues which known as foliar endophyte fungi
It is assumed that endophytic fungi which abundant in our environment could be used as biofertilizer

Summary

INTRODUCTION

Fungi are abundant in our environment, and it’s recorded that there were almost 1.5 million of species fungus were found on the earth (Hawksworth, 2004). Like others fungi which have ability to transform inorganic phosphate to organic phosphate that can help plant growth (Pradhan dan Sukla, 2005; Barrow dan Osuna, 2002), foliar endophyte fungi known to have the same ability (Kauppinen et al, 2016; Cheplick, Clay and Marks, 1989). Regarding these theories, it is assumed that endophytic fungi which abundant in our environment could be used as biofertilizer. The objectives of this research are to isolate foliar endophytic fungi from peatland and to investigate its potential ability to enhance plant growth by in vitro method

MATERIALS AND METHODS

Findings

AND DISCUSSION Exploration of Foliar Endophyte Fungi