Abstract
Snake-head fish (Channa striata) skin categorized a byproduct contains a higher concentration of collagen. The collagen can be extracted by using protease, which is known as Papain Soluble Collagen (PaSC). This study aimed to isolate the collagen from the snake-head fish skin using papain. The yield percentage and moisture content of PaSC in the skin was determined. Measurements using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared spectroscopy (FTIR) were performed to characterize the PaSC and its functional groups. The results showed that the yields and moisture content of PaSC were 8.9% and 6.07%, respectively. The PaSC characterized by two α-chains appeared as collagen type I. Fourier transform infrared (FTIR) spectra of PaSC confirmed a triple-helical structure of collagen. The results indicated that snake-head fish skin could be used as potential resources of collagen and papain can be used as an alternative affordable enzyme.
Highlights
Bovine and pig are major sources of collagen
Yield and moisture content of Papain Soluble Collagen (PaSC) from the skin of snake-head fish The yield percentage of PaSC extracted from the skin of snake-head fish was 8.9%
Compared to other sources of collagen, the obtained yield of PaSC was slightly similar with pepsin soluble collagen (PSC) extracted from the skin of brown-banded bamboo shark (8.86%) [8], but was higher than PSC from striped catfish (7.7%) [7], brownstripe red snapper (4.7%) [1], and bigeye snapper (1.1%) [5]
Summary
Bovine and pig are major sources of collagen. Products contain the collagen sourced from pig are not permitted for consumption under Islamic law. Alternative sources of the collagen, e.g. aquatic animals, are essential to replace the bovine- and pig-source collagen [2]. Snake-head fish is one of the freshwater organisms, categorized as Channidae family. In Indonesia, amount of the snake-head fish being catched increased from 27.930 tonnes to 34.007 tonnes between 2009 and 2010 [3]. The snake-head fish contains a high level of albumin, found in flesh of the fish [4]. The flesh of the fish is dominantly consumed, while other parts of the fish, e.g. bones and skin, are wasted. The skin contains a high concentration of collagen and can be, a potential source of the collagen
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