Abstract
A procedure has been developed for the isolation of a P700-chlorophyll-protein complex from a blue-green alga by a nondetergent method. The use of a Sepharose 4B column in conjunction with sucrose density-gradient centrifugation allows the separation of this complex from three other chlorophyll a-containing fractions which have higher ratios of carotenoids to chlorophyll a. Isoelectric focusing results in a single band with an isoelectric point at 4.5. Analysis of the major (green) fraction reveals the presence of P700. The absorption and emission spectra of this complex are reported.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
