Abstract
A new method is described for the isolation of sequence-specific DNA-binding proteins, based on simultaneous, competitive binding to homologous or heterologous DNA. Since both types of DNA are bound to a solid matrix (Sephadex beads), the method may be used for the preparative isolation of proteins which bind preferentially to specific DNA sequences. Fractionation of nucleolar proteins from Novikoff hepatoma ascites cells by this technique yields two protein fractions which show much greater apparent specificity of binding to homologous DNA, in a nitrocellulose filter-binding assay, the unfractionated nucleolar proteins.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
