Abstract
The peptide omega-agatoxin-IIIA (omega-Aga-IIIA) blocks ionic current through L-type Ca channels in guinea pig atrial cells without affecting the associated gating currents. omega-Aga-IIIA permits the study of L-type Ca channel ionic and gating currents under nearly identical ionic conditions. Under conditions that isolate L-type Ca channel currents, omega-Aga-IIIA blocks all ionic current during a test pulse and after repolarization. This block reveals intramembrane charge movements of equal magnitude and opposite sign at the beginning of the pulse (Q(on)) and after repolarization (Q(off)). Q(on) and Q(off) are suppressed by 1 microM felodipine, saturate with increasing test potential, and are insensitive to Cd. The decay of the transient current associated with Q(on) is composed of fast and slow exponential components. The slow component has a time constant similar to that for activation of L-type Ca channel ionic current, over a broad voltage range. The current associated with Q(off) decays monoexponentially and more slowly than ionic current. Similar charge movements are found in guinea pig tracheal myocytes, which lack Na channels and T-type Ca channels. The kinetic and pharmacological properties of Q(on) and Q(off) indicate that they reflect gating currents associated with L-type Ca channels. omega-Aga-IIIA has no effect on gating currents when ionic current is eliminated by stepping to the reversal potential for Ca or by Cd block. Gating currents constitute a significant component of total current when physiological concentrations of Ca are present and they obscure the activation and deactivation of L-type Ca channels. By using omega-Aga-IIIA, we resolve the entire time course of L-type Ca channel ionic and gating currents. We also show that L- and T-type Ca channel ionic currents can be accurately quantified by tail current analysis once gating currents are taken into account.
Highlights
The spider toxin co-Aga-IIIAblocks currents through L-type Ca channels in cardiac myocytes with no effect on T-type Ca channels (Mintz, Venema, Adams, and Bean, 1991; Cohen, Ertel, Smith, Venema, Adams, and Leibowitz, 1992a)
We show that L- and T-type Ca channel ionic currents can be accurately quantified by tail current analysis once gating currents are taken into account
Single guinea pig atrial myocytes were prepared from male Duncan-Hartley guinea pigs as described previously (Mitra and Morad, 1985; Cohen et al, 1992b)
Summary
The spider toxin co-Aga-IIIAblocks currents through L-type Ca channels in cardiac myocytes with no effect on T-type Ca channels (Mintz, Venema, Adams, and Bean, 1991; Cohen, Ertel, Smith, Venema, Adams, and Leibowitz, 1992a). Several recent studies have indicated that ventricular myocytcs possess a large component of dihydropyridine (DHP)-sensitive intramembranc charge movement, presumably arising from the gating of L-type Ca channels (Field, Hill, and Lamb, 1988; Bean and Rios, 1989; Hadlcy and Ledcrcr, 1991; Josephson and Sperelakis, 1992; Shirokov, Levis, Shirokova, and Rios, 1992). These studies have relied on transition metals such as Cd, Co, and/or La to suppress ionic currents because they lacked high-affinity blockers of Ca channel ionic currcnt. By using t0-Aga-IIIA, one can mcasure ionic and gating currents under nearly identical conditions bccausc the toxin blocks ionic current through L-type Ca channels and has no effect on the associated gating currents
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