Abstract
Introduction:Rapidly growing mycobacteria (RGM) are ubiquitous and are usually considered as saprophytes, and have been recovered from the environment, particularly in dust, watery soil and water distribution systems. However, Mycobacterium massiliense is a rare causative agent of ocular infection.Case presentation:We report a case of M. massiliense in a 44-year-old female with signs and symptoms of a corneal ulcer. We carried out PCR-based DNA sequencing targeting the hsp 65 gene for the identification of M. massiliense. To confirm the identification, we also performed PCR-based RFLP targeting the hsp65 gene and PCR-based DNA sequencing targeting the internal transcribed spacer region, which showed 97 % nucleotide identity with M. massiliense.Conclusion:To the best of our knowledge, this is the first study in India to report the detection of M. massiliense from a corneal biopsy.
Highlights
Growing mycobacteria (RGM) are ubiquitous and are usually considered as saprophytes, and have been recovered from the environment, in dust, watery soil and water distribution systems
To the Abbreviations: ITS, internal transcribed spacer; Rapidly growing mycobacteria (RGM), rapidly growing mycobacteria best of our knowledge, this is the first report on the isolation of M. massiliense from a corneal biopsy in India
Nearly 95 % of soft-tissue infections caused by RGM are M. chelonae–M. abscessus complex infections (Simmon et al, 2007)
Summary
Growing mycobacteria (RGM) are ubiquitous and are usually considered saprophytes, and have been recovered from the environment, in dust, watery soil and water distribution systems. Mycobacterium massiliense is a rapidly growing Mycobacteria species sharing an identical 16S rRNA gene sequence with Mycobacterium abscessus. To the Abbreviations: ITS, internal transcribed spacer; RGM, rapidly growing mycobacteria best of our knowledge, this is the first report on the isolation of M. massiliense from a corneal biopsy in India. PCR-based RFLP (Wang et al, 2004) using the enzymes BstEII and HaeIII targeting. Kulandai and others the hsp gene and PCR-based DNA sequencing targeting the hsp and internal transcribed spacer (ITS) (Telenti et al, 1993) region were performed. Antibiotic susceptibility testing was performed using the Kirby–Bauer method, and the isolate was found to be sensitive to cefoperazone, doxycycline, cefotaxime and clarithromycin and resistant to cefuroxime, ceftriaxone, ceftazidime and cefazolin
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