Isolation of Murine Myeloid Progenitor Populations by CD34/CD150 Surface Markers

Abstract

Myeloid progenitors are intermediates between Hematopoietic Stem Cells and their effector progeny. In mouse bone marrow, they are part of the Linage- Sca1- cKit+ (LK) compartment. Up to date, most researchers used CD34 and FcγR surface markers for dissection of this compartment into various populations. However, this approach does not provide distinct separation by fluorescence-activated cell sorting (FACS). In this study, we re-analyzed previously published single-cell index-sort and RNA-Seq. data and found that dissection of the LK compartment by surface expression of CD34 and CD150 provides better-defined populations of cells compared to the classical CD34/FcγR-based approach. We confirm our finding by FACS, and demonstrate the distinct differentiation potential of the newly-obtained LK sub-populations. Therefore, we suggest an alternative gating strategy for murine myeloid progenitors, utilizing commonly-used surface markers, and highlight the differences between populations based on transcriptome and phenotype.