Abstract
SummaryA virus has been isolated from cattle which displays typical symptoms and pathology of bovine mucosal disease. Virus isolations and propagation were carried on using tissue cultures of trypsinized bovine kidney cells in Mixture 199 exclusively. Two calves which were negative for neutralizing antibodies for bovine mucosal disease were injected with tissue culture virus and developed hyperimmune serum from 15 to 25 days post-inoculation. Serology conducted with laboratory rabbits indicate similarity of viruses isolated from 4 separate herds of cattle in North Dakota.
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