Abstract
Mangifera indica (M.indica) is a well-known plant for its medicinal value. Different parts of M. indica have been scientifically proved to possess medicinal properties. Anti-biofilm activity of Mango twigs extract (MTE) and its biomolecule, methyl gallate (MG) have been explored against clinical pathogenic bacteria, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus. Extract was prepared using ethyl acetate. Methyl gallate was isolated from ethyl acetate extract using column chromatography. Structure of methyl gallate was established using spectral techniques. The anti-biofilm activity was tested using static crystal violet staining method and visualized by stereo zoom light microscopy. At the concentration of 5 and 8 mg/ml of ethyl acetate extract and methyl gallate were disrupts and inhibits the formation of biofilm. The present findings proved that the mango twigs and methyl gallate have got the potential to inhibit the formation of biofilm. It could be used for preparation of neutraceuticals as potent antibacterial to treat various biofilm related human diseases.
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