Isolation of Mammary Epithelial Cells and Fibroblasts From Mouse Tumor

Abstract

Developing cancer therapeutics requires the ability to investigate their effects using in vitro models of a specific type of tumor. This protocol provides a method for the isolation and adoption to growth in culture of cells from primary tumors. This is particularly valuable for studying mouse models where original tumor cells can be evaluated, for example for gene modifications, and subsequently injected back to the same background mice to create more tumors for in vivo efficacy studies.