Abstract
Elevated lysozyme activity was observed in the urine of patients with Itai-itai disease. The urinary lysozyme was isolated from the urine specimens of these patients by gel filtration and ion exchange chromatography. Two lysozyme peaks were found on Sephadex G-100 gel filtration. The first peak (lysozyme-E) had two components on DEAE-cellulose chromatography. One of them showed more acidic chromatographic behavior than the other component which was eluted at the void volume of the column. The second peak (lysozyme-F) on Sephadex G-100 gel filtra-tion had only the component which was similarly eluted at the void volume of the DEAE-cellulose chromatographic column. Slight differences in the chromatographic behavior and in amino acid composition between lysozyme-E and -F indicated that the urinary lysozyme was composed of two components.
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