Abstract

Three Leuconostoc mesenteroides and a Lactobacillus plantarum strains were isolated from kimchi based on their acid and bile salts tolerances and bile salt hydrolase activities. They also possess β-galactosidase (β-Gal) and β-glucosidase (β-Glu) activities. When exposed to pH 3.0 for 3 h, Lb. plantarum C182 showed the highest survival ratio (52.50%). Lb. plantarum C182 was also the most resistant against 0.3% bile salts (58.53%) followed by Leu. mesenteroides C10 (42.85%), F27 (41.17%) and C4 (30.41%). All strains were sensitive to ampicillin, chloramphenicol, cyclohexamide, erythromycin, neomycin, streptomycin, tetracycline, and rifampicin, but resistant to vancomycin. Three Leuconostoc strains adhered to HT-29 cells (16.3–29.6 CFU/cell) better or similar with Lb. rhamnosus GG (17.1 ± 0.6 CFU/cell). These results indicate that 4 strains might be useful as probiotics.

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