Isolation of L Type Growth from a Strain of Bacteroides funduliformis.

Abstract

It has been described in a previous note that in cultures of certain bacterial strains belonging to various species tiny colonies, similar to young colonies of the pleuropneumonia group of organisms develop.1 During the last year these colonies were isolated in pure culture from a strain of flavobacterium and one of Bacteroides funduliformis respectively. The colonies isolated from the flavobacterium always died out after 2 or 3 transplants; those isolated from the funduliformis apparently can be indefinitely maintained in cultivation. Colonies resembling those of the organism of pleuropneumonia bovis were first isolated by Klieneberger from cultures of Streptobacillus moniliformis.2 She designates them with the letter L. Recently she described the isolation of similar cultures from a Gram negative streptobacillus causing suppurative lesions in guinea pigs.3 The characteristics which differentiate these L type colonies from the usual bacterial colonies are the following:The colonies are very tiny. The gro...