Isolation of Keratinolytic Microorganism Streptomyces chartreusis strain ISP 5085 from the Soil Sample: Characterization, Degradation and Microbial Identification using 16S rDNA Region

Abstract

Keratinolytic microorganisms have a broad significance in feather waste degradation and it can be used for advancement of livestock feed and production of protein hydrolysates. Production of keratin into the environment in different forms is very high which leads to setback for its degradation. An approach for degradation of keratin based wastes through potential microorganism with higher efficiency can be attained. The study shows that maximum DFD% i.e. Degree of Feather (keratin) Degradation, through the isolates is achieved, when nutrient medium is kept at temperature 30 °C and pH-8.5 supplemented with dehydrated powdered form of chicken feathers as keratin source for 72 h – 120 h. Chicken feathers acts as a better substrate and source of keratin content. A molecular approach was necessary therefore; phylogenetic trees of the isolate were constructed with neighbour-joining method through the Microbial Identification using 16S rDNA Region, which showed that sequence exhibited a high level of homology with Streptomyces chartreusis strain ISP 5085. The present study deals with identification of isolates that play a significant role in the degradation of chicken feather waste as well as keratin degradation ability of the isolates and optimum conditions required for it.