Abstract
Highly efficient hydrogen-producing anaerobic microflora was enriched from anaerobic sludge obtained from a methane fermentation plant, with soluble starch as substrate. The effect of culture medium pH on hydrogen production capacity and microbial community was investigated using a batch reactor equipped with a pH controller. The optimal pH was 5.0, the hydrogen yield was 2.59 mol/mol-glucose, and the maximum hydrogen production rate was 123 mL/L-culture/h. Partial 16S rDNA sequencing placed the isolated strain within the Clostridiaceae family. When the isolated strain adhered to polymer resin, the change in free energy of interaction was negative, indicating that the bacteria-resin interaction is thermodynamically favorable, and that the strain will readily immobilize on the resin.
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