Abstract
High quality DNA is essential for many molecular biology techniques. However, the reagents used for that purpose usually are expensive and/or cause a high environmental impact. Here, we describe two alternative protocols that use inexpensive reagents and are not hazardous to the environment. The first utilizes the enzyme chymosin, normally used as in cheese production and which is easily obtained on the commercial market. The second uses rennet DNA extraction protocol combined with the DNA binding capacity of glass powder (glass milk), which can easily be home made. The first is used when a high yield of DNA is needed, whereas the second is used for production of a higher quality DNA, being able to work with sparse samples.
Highlights
High quality DNA is essential for many molecular biology techniques
The first protocol utilizes the enzyme chymosin, normally used as “rennet” in cheese production and which is obtained on the commercial market
The first protocol is used when a high yield of DNA is needed, whereas the second protocol is used for production of a higher quality DNA, being able to work with sparse samples
Summary
The combination of both protocols is useful to solve problems related to the quality of DNA extracted from some plants, since this is generally associated with secondary metabolites or latex. In the first protocol, which is chymosin-based, a work solution of calf rennet is prepared at 0.25 g/ml and maintained at -20oC. 60 μl of the rennet work solution is added and the tube is maintained in a water bath at 37oC for an additional hour.
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