Abstract
Frankia is a slow-growing, Gram-positive, nitrogen-fixing filamentous actinobacterium that forms a symbiotic association with actinorhizal plants, but it can also be found free-living in soil. In the symbiotic association, Frankia is an important contributor to nitrogen fixation. Actinorhizal root nodules will collate and surface-sterilize with 10–20% hydrogen peroxide and the root nodules to crush and dry for dehydrating. One loop of the crushed sample has been directly inoculated on the center of the defined propionate minimal (DPM) medium and broth. The plates and flasks will be inoculated at optimum temperature at 28 °C and allowed 12–15 days for growth budding.
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