Abstract
<p class="Els-Abstract-text"><em>Phalaenopsis amabilis </em>(L.) Blume<em> </em>is an indigenous orchid species in Indonesia. This orchid has a white large flower. The large flower is caused by the existence of gene that has an important role in flower development. One of the genes is <em>SEPALLATA 1. </em>This gene is a member of superfamily MADS-Box gene. <em>SEPALLATA 1 </em>gene is a marker of primordial flower organ. This study aimed to isolate <em>SEPALLATA1</em><em> </em>gene from <em>Phalaenopsis amabilis </em>(L.) Blume by PCR using forward primer 5’-GCT-GGA-GCG-GAT-CGA-GAA-CA-3’and reverse primer 5’-TCA-TGC-AAG-CCA-ACC-AGG-TG-3’<em>. </em>This study successfully amplified 691 bp lengths of <em>SEPPALATA1</em> fragment, lacking 20 bp upstream which consist its start codon.</p><p> </p><div><p class="Els-keywords"><strong>Keywords:</strong> Flower development regulation, <em>Phalaenopsis amabilis</em> (L.) Blume, SEPALLATA 1 gene.</p></div>
Highlights
Phalaenopsis amabilis (L.) Blume is a member of the family Orchidaceae
Phalaenopsis amabilis (L.) Blume samples were collected from DD’ Orchid Nursery, Dadaprejo, Batu
The acquired DNA sequence compared to SEP1 gene from P. equestris to determine their similarity index
Summary
Phalaenopsis amabilis (L.) Blume is a member of the family Orchidaceae. This orchid has unique flower form [1, 2]. The sepal is white-colored like the petal, but it has a different form. The sepals are ellipse to acute shaped while the petals are widened circular with a small base and dull top, one of petal modified to be labellum form. The labellum has a pale yellow color to dark yellow with red stripes on the inside [3, 4]. There are stamen and pistil in the gynostemium [5]
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