ISOLATION OF FLAVONOID XANTHINE OXIDASE INHIBITORS FROM KNOTWEED HERB. CHEMICAL NA-TURE AND MECHANISM OF ACTION

Abstract

The aim is to isolate flavonoid xanthine oxidase (XO) inhibitors from the herb of Knotweed, to characterize their nature and to determine the mechanism of action inhibitors.
 Objectives: 1. To isolate the amount of flavonoids from the herb of Knotweed and to assess their effect on the activity of XO. 2. Purify the total extract and obtain active fractions containing XO inhibitors, identify their nature. 3. Determine the mechanism of inhibition of XO, isolated by flavonoids.
 Methodology: Grass P. hydropiper extracted with 90% ethanol (70 °C for 1 h). A native extract obtained with a flavonoid content of 1.51%. The extract fractionated with heptane and ion exchange chromatography. The anti-xanthine oxidase activity of the fractions assessed by the spectrophotometric method. KO activity expressed in IU / g. The mechanism of action of KO inhibitors in the fractions was determined by constructing kinetic curves of the enzymatic reaction and calculating Km. Flavonoids were identified by TLC, PC, spectrophotometry.
 The obtained results and conclusions: 1. The sum of flavonoids from the herb of Knotweed inhibit the activity of KO at a concentration of 2.5 mg / ml. 2. Chalcones inhibiting XO were isolated from the native extract of the herb of Knotweed. 3. Flavonoids in the active fraction reversibly inhibit milk XO by a competitive mechanism
 Practical significance and directions for further research: The information obtained is the basis for the development of a new drug used to treat gout. It is necessary to develop a method for isolating a homogeneous flavonoid and conduct its pharmacokinetic studies.