Abstract
Eatrone, estradiol-17β and estriol were isolated and identified from the urine of normal menstruating women, and from a case of secondary amenorrhea with apparently no increased urinary PSH. Quantitative estimation by fluorometry, coupled with tracing of added labelled estrone and estradiol-17β, throughout the various stages of the isolation procedure, confirmed the reliability of the fluorometric method. Neither of the estrogens could be isolated from urine of a patient with secondary amenorrhea with increased PSH excretion, nor from a patient with bilateral ovarieotomy. In both cases the fluorometric values were close to zero.
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