Abstract
Survey of rotavirus was conducted in 40 fecal samples which were divided into 4 groups according to diarrheal conditions to clarify the relationship between rotavirus and foals with diarrhea. Particles characteristic of rotavirus were directly detected by electron microscopy from 5 of 14 fecal samples collected from foals with acute diarrhea. Moreover, rotaviruses were isolated from 4 of 5 fecal samples with MA-104 cells and with trypsin treatment, and were capable of being successively passaged in these cells. On the other hand, no rotavirus was detected in 26 fecal samples collected from foals with various non-acute diarrhea. In the cross serum neutralization test, 4 isolated strains were antigenically recognized as the same serotype, which was slightly differed from BI strain of equine rotavirus and clearly separated from Lincoln strain of bovine rotavirus antigenically. From these results, it was revealed that rotavirus was closely related only to acute diarrhea in foals. Moreover, it was confirmed that equine rotavirus in field cases could be isolated in MA-104 cells with trypsin treatment.
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