Isolation of endothelial progenitor cells from human adipose tissue

Hoa Trong Nguyen,Ngoc Bich Vu,Ngoc Kim Phan,Phuc Van Pham

doi:10.15419/bmrat.v3i05.98

Hoa Trong Nguyen, Ngoc Bich Vu + Show 2 more

Open Access

https://doi.org/10.15419/bmrat.v3i05.98

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Abstract

Adipose tissue is a rich source of stem cells, especially mesenchymal stem cells (MSCs). This study aimed to identify and isolate endothelial progenitor cells (EPCs) from human adipose tissue. Belly adipose tissues were collected from donors with consent. Stromal vascular fractions (SVFs) were extracted from adipose tissues by enzyme collagenase using commercial kits. SVFs were cultured in MSCCult medium for 24 h to obtain MSCs, then supernatant was collected and cell pellet cultured in EGM-2 medium to obtain adipose tissue EPCs (ADEPCs). ADEPCs were checked for surface marker expression of CD31 and VEGFR2, and for angiogenesis capability in vitro. The results showed that SVFs contained a pool of EPCs with strong angiogenesis potential and that adipose tissue is not only a source for MSCs but also for EPCs. Therefore, ADEPCs may a useful source of EPCs for vascular medicine.

Highlights

Diseases related to ischemia have gradually increased in recent years
The greatest limitation of endothelial progenitor cells (EPCs)-based transplantation is the scarcity of EPCs; unlike other stem cells, EPCs exist in umbilical cord blood (Finney et al, 2006; Lin et al, 2011; Moon et al, 2013; Phuc et al, 2012), bone marrow (Ii, 2010) and peripheral blood (Donndorf et al, 2015), with extremely low numbers, and have slow proliferation in vitro
The results showed that adipose tissues contained 1.31±0.27 106 Stromal vascular fractions (SVFs) cells with percent viability of 92.17±5.81%

Summary

Introduction

Diseases related to ischemia have gradually increased in recent years. There have been many therapeutic efforts to treat ischemic heart diseases, including surgery (angioplasty, stents, atherectomy, etc.) to medications (beta-blockers, blood thinners, diuretics, etc.) (Imai et al, 2016; Kang et al, 2016; Lukasiewicz, 2016; von Segesser et al, 2016). The greatest limitation of EPC-based transplantation is the scarcity of EPCs; unlike other stem cells, EPCs exist in umbilical cord blood (Finney et al, 2006; Lin et al, 2011; Moon et al, 2013; Phuc et al, 2012), bone marrow (Ii, 2010) and peripheral blood (Donndorf et al, 2015), with extremely low numbers, and have slow proliferation in vitro To overcome these limitations, our study is aimed at isolating and culturing EPCs from adipose tissue, as an alternative primary source

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