Abstract
This study designed to isolation virulence E. coli, specific phages and identify in rabbits biochemically, serologically, detect virulence genes using PCR and susceptibility to antimicrobial range. For propose fifty-fecal-swab samples collected from diarrheic and freshly-dead-young-rabbits were presented to bacteriological examination. Data showed twenty-seven samples (54%) gave positive results of Escherichia coli. Data in-vitro Pathogenicity-test using Congo-red(CR) binding assay showed eight E. coli isolates (29.63%) were Congo-red positive. Antimicrobial susceptibility to several antibiotics was studied. E. coli isolates showed different susceptibility degrees to antibiotics, and isolates 3,4 and 8 categorized as multidrug resistant isolates. Three selected E. coli isolates were serologically identified classified as O169, O125 and O158 serotypes for isolates 4,8,3 respectively. Conventional polymerase chain reaction for detection eaeA and Stx1 virulence genes revealed E. coli serotypes O158 expressed eaeA gene, but others, O169 and O125 don't expressed. While, none serotypes expressed Stx1 gene. Phages may be used effectively to control of pathogenic E. coli, colonizing farm rabbit's intestines. However, harsh acidic-conditions and digestive enzymes activities influence phage infectivity, and decrease efficiency in application-trails. Natural-defensive-barrier development was being suitable for oral administration to farm poultry presented acid-stability. Encapsulated pahge beads in gelatin chitosan -matrix showed partial phage titer reductions. Phage beads titers were constant for storage in water, but complete release achieved after 6hr in simulated intestinal solution at 37°C. Finding multidrug resistant enterohaemorrhagic, E. coli serotypes created severe health hazard for rabbits and contact human. Encapsulated phages beads are promising and cost-effective method for bacteriophage targeting intestinal bacteria of farm-rabbits.
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