Abstract

BackgroundThis study examined the quality of bone marrow aspirates extracted using a novel, FDA cleared method to optimally target cells from the inner cortical iliac bone surface without the need for centrifugation. This method employs small draws from a single puncture that promote only lateral flow from multiple sites (SSLM method). The study utilized the Marrow Cellutions bone marrow aspiration system (MC system) which is based on the SSLM method and compared the MC system directly to bone marrow concentrates (BMAC) generated by centrifugation of aspirates harvested with a standard aspiration needle.MethodsThree direct comparisons were conducted evaluating the SSLM draws and BMACs derived from the same patient from contralateral iliac crests. The levels of TNCs/mL, CD34+ cells/mL, CD117+ cells/mL, and CFU-f/mL were compared between the various bone marrow preparations. The cellular content of a series of SSLM draws was also analyzed to determine the total nucleated cell (TNC) count and the concentration of mesenchymal stem/progenitor cells as measured by colony forming unit fibroblasts (CFU-f).ResultsIn direct comparisons with BMAC systems, SSLM draws yielded significantly higher CFU-f concentrations and comparable concentrations of CD34+ and CD117+ cells. In addition, the average quantity of TNCs/mL in a series of 30 patients utilizing the SSLM draw was 35.2 × 106 ± 17.1 × 106 and the average number of CFU-f/mL was 2885 ± 1716. There were small but significant correlations between the TNCs/mL and the CFU-fs/mL using the SSLM method as well as between the age of the patient and the CFU-fs/mL.ConclusionsThe MC Device, using the SSLM draw technique, produced concentrations of CFU-fs, CD34+ cells and CD117+ cells that were comparable or greater to BMACs derived from the same patient. Given the rapid speed and simplicity of the MC Device, we believe this novel system possesses significant practical advantages to other currently available centrifugation based systems.

Highlights

  • This study examined the quality of bone marrow aspirates extracted using a novel, FDA cleared method to optimally target cells from the inner cortical iliac bone surface without the need for centrifugation

  • We found that the Marrow Cellution (MC) system produced concentrations of colony forming unit fibroblasts (CFU-f), CD34+ cells and CD117+ cells that were comparable or greater to bone marrow concentrates (BMAC) derived from the same patient

  • In order to better characterize the aspirates generated by the MC system, we examined the correlation between total nucleated cell (TNC) and CFU-f counts obtained from MC system aspirates

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Summary

Introduction

This study examined the quality of bone marrow aspirates extracted using a novel, FDA cleared method to optimally target cells from the inner cortical iliac bone surface without the need for centrifugation. This method employs small draws from a single puncture that promote only lateral flow from multiple sites (SSLM method). Bone marrow aspirate (BMA) and concentrates (BMAC) have been used to treat a variety of orthopedic injuries as well as critical limb ischemia and heart failure with positive clinical outcomes being linked to higher numbers of stem/progenitor cells in the bone marrow sample [1,2,3,4,5,6,7]. While BMAC systems can increase the concentration of nucleated cells in the aspirate [11], because they do not distinguish between peripheral blood and marrow components, they may merely increase the level of peripheral blood nucleated cells, which contain very few stem/progenitor cells

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