Abstract
Veterinaria México OA ISSN: 2448-6760Cite this as:Mora-Díaz JC, Díaz-Aparicio E, Herrera-López E, Suárez-Güemez F, Escalante-Ochoa C, Jaimes-Villareal S, et al. Isolation of Chlamydia abortus in dairy goat herds and its relation to abortion in Guanajuato, Mexico. Veterinaria México OA. 2015;2(1).doi: 10.21753/vmoa.2.1.339.Although Chlamydia abortus is classified as an exotic agent in Mexico, there is increasing evidence of its presence. The objective of this study was to isolate C. abortus in dairy goat herds with problems of abortion in the state of Guanajuato, Mexico, and to develop appropriate diagnostic methods for its detection. Serological samples and vaginal swabs were taken from 6 dairy goat herds. The ELISA revealed a seropositivity of 9.60% for C. abortus. The PCR test based on the vaginal mucus samples resulted in 30 of 126 positive animals (23.8%). Chlamydia spp. were isolated in 34 of the 126 animals tested (26.98%). The 3 diagnostic methods tested were valuable and complementary in zones where Chlamydia is suspected to cause abortions. We demonstrated that the bacteria are present in dairy goat herds of Mexico; thus, Veterinary Sanitary Authorities should consider this disease endemic to establish sanitary procedures to control the spread of the disease and to prevent human transmission.Table 2. A comparison of 3 diagnostic tests for small ruminant enzootic abortion. The PCR and bacterial isolation were performed from vaginal swabs of goats that had recently given birth or from aborted goats. The ELISA was conducted with the commercial IDEXX Chlamydiosis Verification Test kit (IDDEX Laboratories Inc.).
Highlights
Aislamiento de Chlamydia abortus en rebaños caprinos lecheros y su relación con casos de aborto en Guanajuato, México
El diagnóstico serológico se realizó con el estuche comercial “IDEXX Chlamydiosis Verification Test”, el cual utiliza un antígeno recombinante y permite detectar la proteína de membrana externa de 80-90 kDa; ésta es específica de C. abortus y no cruza con los antígenos de Chlamydia pecorum
Chlamydophila abortus serological determination in dairy goats in Mexico: preliminary advances
Summary
Publicación Digital de la Facultad de Medicina Veterinaria y Zootecnia http://veterinariamexico.unam.mx oa Artículo Científico. Aislamiento de Chlamydia abortus en rebaños caprinos lecheros y su relación con casos de aborto en Guanajuato, México. Debido a que el AEPR se considera una enfermedad exótica en México, no se cuenta con los reactivos, anticuerpos y técnicas de diagnóstico que permitan la rápida identificación de un caso donde C. abortus esté involucrada. El objetivo del presente estudio fue determinar la presencia de C. abortus mediante aislamiento bacteriológico, Reacción en Cadena de la Polimerasa (PCR, por sus siglas en inglés) y Ensayo por Inmunoabsorción Ligado a Enzimas (ELISA, por su acrónimo en inglés), en rebaños caprinos lecheros con problemas de abortos sugerentes de clamidiosis, en el estado de Guanajuato, México, y establecer los métodos diagnósticos apropiados para su detección
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