Abstract
Within the frames of activities attributed to the Reference Center for tularemia monitoring at SRC AMB, genetically identified are 4 isolates of Francisella tularensis , isolated in 2011 in the Altai Territory. These bacteria prove to be virulent for BALB/c mice, DCL being lower than 10 CFU. Using single-primer PCR-typing and MLVA assay distinguished have been the subspecies of the isolates. Three of them refer to the Central Asian subspecies, one – to the Holarctic, the former being isolated in the territory of the Russian Federation for the first time ever.
Highlights
State Research Center of Applied Microbiology and Biotechnology, Obolensk; Center of Hygiene and Epidemiology in the Altai Territory, Barnaul
Within the frames of activities attributed to the Reference Center for tularemia monitoring at SRC AMB, genetically identified are 4 isolates of Francisella tularensis, isolated in 2011 in the Altai Territory
These bacteria prove to be virulent for BALB/c mice, DCL being lower than 10 CFU
Summary
Культивирование бактерий F. tularensis проводили при температуре 37 °С на среде FT-агар (ФБУН ГНЦ ПМБ) с добавлением полимиксина В (Pm). Для определения чувствительности к эритромицину культуры высевали на среду, содержащую эритромицин 50 мкг/мл. Выделение ДНК осуществляли методом нуклеосорбции на силикагеле в присутствии гуанидинтиоцианата с использованием коммерческого набора «Рибо-сорб» («ИнтерЛабСервис») из биомассы суточной агаровой культуры. Мышей (по 5 в группе, самцы и самки, возраст 6–8 нед., массой 18–20 г) инфицировали подкожно бактериальной суспензией в дозах 1·101, 1·102 и 1·103 КОЕ/мышь и наблюдали в течение 21 дня. Для VNTR-анализа использовали 25 пар праймеров (Ft-M1 – Ft-M25), последовательности которых приведены в статьях [6, 8, 11]. Japonica mediasiatica mediasiatica novicida holarctica holarctica holarctica holarctica holarctica holarctica holarctica holarctica holarctica holarctica holarctica holarctica. 503 Miura Jasoe 117 120 112 I-282 I-305 I-329 I-346 I-347 I-349 I-365 I-367 I-373 I-382 I-387 I-388
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