Isolation of cDNA, genomic organization and expression of small androgen receptor-interacting protein 1 (PmSARIP1) in the giant tiger shrimp Penaeus monodon

Abstract

The full-length cDNA and genomic organization of small androgen receptor-interacting protein 1 (SARIP1; also called RWD-containing protein 1, Rwdd1) in the giant tiger shrimp (Penaeus monodon) was isolated and characterized. PmSARIP1 was 1366bp in length containing an open reading frame (ORF) of 738bp corresponding to a polypeptide of 245 amino acids. Its genomic sequence contained 5 exons, 4 introns and untranslated regions (UTRs) spanning 5937bp in length. Tissue distribution analysis indicated that PmSARIP1 was specifically expressed in gonads (ovaries>testes) but not in other tissues of wild P. monodon adults. The expression level of PmSARIP1 was not differentially expressed during ovarian maturation in intact wild adults (P>0.05). Eyestalk ablation resulted in up-regulation of PmSARIP1 throughout the ovarian maturation of wild adults where the peak level was observed at stage I (previtellogenic) ovaries (P<0.05). PmSARIP1 mRNA was clearly localized in ooplasm of previtellogenic oocytes. Serotonin injection (5-HT, 50μg/g body weight; 18-month-old shrimp) promoted the expression level of ovarian PmSARIP1 at 6–72h post injection (hpi) with the peak level at 12hpi (P<0.05). Exogenous progesterone administration (0.1μg/g BW; 14-month-old shrimp) did not significantly affect the expression level of ovarian PmSARIP1. In contrast, 17β-estradiol treatment (0.01μg/g BW) resulted in an increase of PmSARIP1 in 14-month-old shrimp at 7days post injection (dpi). Results from this study suggested that PmSARIP1 should play an important role during ovarian maturation of P. monodon.