Abstract
A method is described for the preparation of relatively pure sarcotubular membranes from rat skeletal muscle which have a high capacity to sequester Ca ++. It involves the subfractionation of crude muscle microsomes by the sequential use of two sucrose density gradient centrifugation systems. Subfraction SF 1 contains membranous vesicles which on the basis of both morphological and functional evidence appear to be as free of other cellular components as those obtained from rabbit skeletal muscle microsomes by currently available fractionation methods.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
