Abstract
2,3,5,4'-tetrahydroxy stilbene-2-O-ß-D-glucoside (THSG) exerts multiple pharmacodynamic actions, found in <em>Fallopia multiflora</em>, but the biosynthesis pathway of THSG is still unclear. To clear this ambiguity, we constructed suppression subtractive hybridization (SSH) libraries to screen the genes involved in THSG biosynthesis from two <em>F. multiflora</em> varieties, which vary significantly in THSG content. Twelve non-redundant differentially expressed sequence tags were obtained and the full lengths of 4 unreported fragments were amplified by rapid amplification of cDNA ends. We totally got 7 full-length transcripts, and all of them were aligned to the transcriptome and digital gene expression tag profiling database of four <em>F. multiflora</em> tissues (root, stem and leaf from Deqing <em>F. multiflora</em> and another root from Chongqing <em>F. multiflora</em>; data unpublished) using local BLAST. The results showed that there was a significant, organ specific difference in the expression of fragments and full-length sequences. All the sequences were annotated by aligning to nucleotide and protein databases. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that THSG biosynthesis was correlated with multiple life activities.
Highlights
Fallopia multiflora is a traditional Chinese medicinal herb, which has been widely used for thousands of years
Quantification of tetrahydroxy stilbene-2-O-ß-D-glucoside (THSG) To select the specific samples with robust difference in THSG concentration, HPLC was used to analyze the THSG contents
As of any secondary metabolite in plant, the production of THSG in F. multiflora is influenced by multiple factors, such as temperature, climate, altitude, precipitation etc
Summary
Fallopia multiflora is a traditional Chinese medicinal herb, which has been widely used for thousands of years. 2,3,5,4'-tetrahydroxy stilbene-2-O-ß-Dglucoside (THSG) is an active component in F. multiflora, which possesses anti-hyperlipidemic [4], anti-oxidative, anti-inflammatory, endothelial-protective activities [5]. Handling Editor: Przemysław Wojtaszek disorders such as Parkinson’s disease, showed that THSG may protect neurons against MPP+- induced cell death through improving mitochondrial function, decreasing oxidative stress and inhibiting apoptosis [8]. THSG and resveratrol (3,5,4'-trihydroxy-trans-stilbene) belong to phenylpropanoids characterized by a 1,2-diphenylethylene backbone. Plant stilbenes are derived from phenylalanine biosynthesis via the general phenylpropanoid pathway. One p-coumaroylCoA or cinnamoyl-CoA derived from the phenylpropanoid pathway is ligated with three malonyl-CoA under the catalysis of stilbene synthase (STS) and stilbene is the product [9]. Sheng et al isolated a stilbene synthase gene FmPKS from the rhizomes of F. multiflora. The gene expression pattern in the plant correlated with the THSG content in different tissues, but
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
