Abstract
The human oral cavity is host to a complex microbial community estimated to comprise > 700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated.
Highlights
The human oral cavity contains a complex microbial community estimated to comprise 4 700 bacterial species (Kazor et al, 2003; Paster et al, 2006; Dewhirst et al, 2010)
A number of early studies investigating extrachromosomal DNA in bacteria isolated from the oral cavity have reported the presence of small cryptic plasmids in strains of Streptococcus mutans (Dunny et al, 1973; Macrina et al, 1977; Caufield et al, 1982; Bergmann et al, 1990)
This study uses a transposon-aided capture (TRACA) method (Jones & Marchesi, 2007), which is independent of bacterial culture or phenotype selection, to isolate plasmid DNA from dental plaque samples taken from patients with periodontal disease
Summary
The human oral cavity contains a complex microbial community estimated to comprise 4 700 bacterial species (Kazor et al, 2003; Paster et al, 2006; Dewhirst et al, 2010). Keywords oral cavity; plasmid; TRACA; dental plaque; metagenome. A number of early studies investigating extrachromosomal DNA in bacteria isolated from the oral cavity have reported the presence of small cryptic plasmids in strains of Streptococcus mutans (Dunny et al, 1973; Macrina et al, 1977; Caufield et al, 1982; Bergmann et al, 1990).
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