Isolation of Bacteria from Spent Engine Oil Polluted Site in Bali and Its Environs Taraba State, Nigeria

Abstract

Isolation and identification of microorganisms associated with engine oil contaminated soil was carried out. Five different mechanic workshops within Bali and its environs Taraba State were selected and soil samples were collected from each site. Twenty-five grams (25g) of contaminated soil was added to conical flask containing 250 mL of distilled water. The media was prepared by dissolving 3.5g of nutrient agar powder in 125ml of distilled water. The mixture was autoclave at 1210C for 15minute allow to and cool but not solidify. The sterile nutrient agar was aseptically pure plate, 25ml each of the five different petri dish and allowed to solidify. A wire loop was used to transfer amount of spent engine oil polluted soil (stock solution) and inoculated by striking the solidified upper surface layer of the agar plate. The plate was invertically incubated for 24hrs at 370C Singly colonies growing on nutrient agar plate was transferred using wire loop and sub-culture on to freshly prepared culture media (certified agar). The pure culture- plate was inoculated for 24hrs at 370C.The pure culture isolate was identified macroscopically, microscopically and biochemically using standard protocols .Result: The bacterial species isolated were Pseudomonas aeruginosa. Whereas Gazabu has the highest count of the species and Garba Chede has the least.