Isolation of Bacillus aryabhattai GW320 from the Cucumber Rhizospheric Soil and Evaluation of Plant Growth Promoting Activity

Abstract

Currently, as part of eco-friendly agriculture in Korea, useful agricultural microorganisms are provided to farmers through 141 agricultural technology centers nationwide. In particular, there are very few cities and counties agricultural technology centers are using indigenous and patented microorganisms. Thus, this study was aimed to isolate Bacillus aryabhattai GW320 from soil rhizosphere of cucumber cultivation and to estimate the effect on the growth promoting effect of cucumbers. As a result, the GW320 strain showed robust plant growth-promoting rhizobacteria (PGPR) activities such as plant growth promoting hormone, nitrogen utilizing activity, and urea solubilizing activities. Furthermore, in comparison with control group, the strain increased plant height 22 to 33% and leaf width by 7 to 9% in cucumber, and elevated plant inorganic components. In addition, when the results of cucumber growth promotion, plant inorganic components, and soil properties were analyzed, the optimum microbial treatment concentration the isolate GW320 was found to be the best when the stock solution was diluted 100 times, and 1.0 × 107CFU mL-1. Concluding remark of this study is that this indigenous strain isolated from rhizosphere of cucumber in Gunwi-gun promoted growth of cucumber, suggesting the importance of indigenous bacterial strain scientifically confirmed in terms of eco-friendly treatment in agricultural field. Various growth parameters of cucumber after 4 weeks according to treatment of B. aryabhattai GW320.