Isolation of Asperigllus flavus and detection of total aflatoxins residues in feed by HPLC

Abstract

Mycotoxins are natural secondary toxic anti nutritive metabolite products of fungi, that contaminate the food stuff , high performance liquid chromatography (HPLC) is one of chromatographic techniques that mainly used in quantitative and qualitative of most mycotoxin residues . A total of 100 random samples of yellow corn and silage feed samples were collected from farms in Ismailia and Suez governorates to detect total aflatoxins residues. Aspergillus flavus were isolated from 86 % , 80 % of yellow corn and silage feed samples. Total aflatoxins residues were detected by HPLC with the means average of Aflatoxin B1, G1, B2, G2 and total aflatoxins residues respectively, in examined 50 yellow corn feed samples were 74.66±12.53 µg/kg, 30.14±6.65 µg/kg,16.13± 2.15 µg/kg ,7.42± 3.28 µg/kg and 85.89 ± 34.94µg/kg. While in examined silage feed samples the mean average were 85.18 ± 17.48 µg/kg,24.02 ± 4.81 µg/kg, 11.79± 2.25 µg/kg, 5.20±1.65 µg/kg and 82.52 ± 18.77 µg/kg. HPLC is suitable technique for the quantification of aflatoxins because of its accuracy and high sensitivity