Abstract
Fusarium wilt disease on banana has been known as panama disease one of the main diseases that cause huge losses for banana farmers. It is caused by the soil-borne fungal pathogen, Fusarium oxysporum f.sp cubense (Foc), which is very hard control because it is saprophytic in the soil. The mold infiltrates the root to vascular tissue that induces yellowing on the leaf, so this pathogen can attack the root, stem dan leaf. The research aimed to search bacteria from the banana rhizosphere that have an antifungal activity to inhibit Foc growth. Bacteria was isolated by serial dilution then was spread on King’s B agar medium incubation 28oC (room temperature). Four quadrants in vitro test on PDA medium used twenty bacterial from isolation, from the test was obtained six isolates have the potential to inhibit the growth of Foc. Based on percentage inhibition radial growth four isolates that have inhibition 50% over which TR2 was the highest at 79.07%. The in vitro test confirmed that bacteria from the banana rhizosphere have potential as biocontrol agent because it was able to inhibit the Foc growth.
Highlights
Pendahuluan Budidaya pisang tidak terlepas dari adanya interaksi dengan lingkungan, organisme dan mikroorganisme lain baik yang menguntungkan ataupun merugikan
Fusarium wilt disease on banana has been known as panama disease one of the main diseases that cause huge losses
It is caused by the soil-borne fungal pathogen
Summary
Kandidat bakteri penghasil antifungi diisolasi dari tanah rizosfer dan rizoplan pisang. Pengambilan sampel dilakukan dengan menggali hingga terlihat akar tanaman pisang kira-kira 15 cm. Sampel yang telah diperoleh dimasukkan ke dalam cool box. Untuk tanah rizosfer dimulai dengan menimbang 1 gr sampel tanah lalu dilarutkan dalam 9 mL buffer posfat. Suspensi tanah pada tingkat pengenceran [, , ] diambil sebanyak 100 μL lalu disebar pada media agar King’s B dan diinkubasi pada suhu 28oC. Sementara itu suspensi rizoplan diperoleh dengan memasukkan 10 gr akar berserta tanah yang melekat ke dalam 100 mL buffer posfat lalu diletakkan pada alat pengaduk selama 10 menit 180 rpm, sebanyak 100 μL dari suspensi yang diperoleh disebar pada media King’s B. Bakteri yang diperoleh dikultur murni pada media King’s B
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