Isolation of Anti-Algal Pseudomonas stutzeri Strains and Their Lethal Activity for Chattonella antiqua

Abstract

Three bacterial strains, A41, B47, and MM4, isolated from sea sediment and aeration tanks of a sewage plant, identified as Pseudomonas stutzeri, showed lethal activity against the red tide phytoplankton Chattonella antiqua. Upon the addition of the culture broth of bacteria, C. antiqua lost its mobility and lysed. Their lethal activity reached a maximum in the nutrient broth containing 3% NaCl at 25°C (the lowest lethal concentration; 0.5%) for 2 to 6 days of cultivation. Addition of the A41 culture broth to a coculture of C. antiqua and killifish (Oryzias latipes) showed the selective toxicity on C. antiqua.