Abstract
Skin aging results in an imbalance between synthesis and degradation of the extracellular matrix. Overproduction of degradative enzymes (MMPs) and oxygen free radicals during chronological and photo-induced aging leads a degradation of the network and elastic skin collagen. Our previous work demonstrated that a culture supernatant of gingival stem cells had anti-aging activities in vitro, ex vivo and in vivo in humans. However, this culture medium is very complex and currently not responding to the European cosmetics regulation. After the analysis of the culture medium we have isolated 4 molecules interesting in terms of skin anti-aging activity: TIMP1, Selenium, Folic Acid and Glycin (TSAG). After the study of the most efficiency concentration of each molecule, the composition of TSAG is tested on irradiated UVA human dermal fibroblast and human skin. TSAG preserves the elastin and collagen network and inhibits the MMP1, MMP3 and MMP9 activities. The advantage of this mimetic solution of stem cells is to be stable, easily reproducible and non-human origin. The outlook for cosmetology seems interesting.
Highlights
Skin aging is associated with an increase in the number and depth of wrinkles as a result of the degradation of macromolecules of the dermis with collagen and elastin
Our previous work has shown that the culture supernatant of human gingival fibroblasts, that have exceptional healing capacity, inhibits the degradation of human skin subjected to UV exposure [2]
3) Determination of the concentration of TIMP1 of TSAG on hDFi cultures: anti-metalloproteases activities (Figure 2) After the Human dermal fibroblast (hDF) irradiation amount of MMP1 and MMP3 secreted by the cells are multiplied by 6 (respectively (a)
Summary
Skin aging is associated with an increase in the number and depth of wrinkles as a result of the degradation of macromolecules of the dermis with collagen and elastin. This is true in the sun-exposed areas, such as facial skin; where the deleterious effects of ultraviolet radiation: collagen imperfect synthesis, skin pigmentation and solar elastolysis result in degradation of skin elastic network. After a careful analysis of the cell culture supernatant, four molecules found in the supernatant have a major activity in skin aging. These molecules are known for their antioxidant, anti-free radicals and anti MMPs. These molecules are known for their antioxidant, anti-free radicals and anti MMPs They stimulate the synthesis of macromolecules including collagen and elastin. TSAG product has been tested in vitro on skin fibroblasts and ex vivo human skin biopsies irradiated with ultraviolet A
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