Abstract
AbstractA method for the isolation of milligram quantities of 3 minorAlternaria mycotoxins, altenuisol (AS), altertoxin I (AT‐I) and altertoxin II (AT‐II) was developed. Crude toxin preparation was first subjected to a preparative high pressure liquid Chromatograph step using PrepLC/System 500 and a silica gel column. The 3 minor toxins were eluted from the column with 25% ethyl acetate. Further purification of these toxins was achieved by passing the partially purified toxins through a Sephadex LH‐20 column (2.5 X 25 cm) twice. In a solvent system of hexane/methylehe chloride/methanol (1:1:1, v/v/v), AT‐I was eluted from the Sephadex column first, followed by AT‐II and AS. The distribution of variousAlternaria mycotoxins in different fractions obtained from the Sephadex step and some of the physicochemical properties of AT‐I and AT‐II are described.
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