Isolation of Aflatoxin B1 from Moldy Foods by Solid-Phase Extraction Combined with Bifunctional Ionic Liquid-Based Silicas.

Luwei Fang,Wei Xiao,Minglei Tian,Xuemin Yan

doi:10.1155/2018/8427580

Luwei Fang, Wei Xiao + Show 2 more

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https://doi.org/10.1155/2018/8427580

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Abstract

A solid-phase extraction method was developed by using new bifunctional ionic liquid-based silicas as sorbents to isolate aflatoxin B1 from moldy corn and peanut. Firstly, according to the adsorption efficiency, two sorbents imidazolium chloride-butylimidazolium chloride-based silica (Sil@BIm-Im) and imidazolium chloride-hexylimidazolium chloride-based silica (Sil@HIm-Im) were selected. The RSM was introduced to optimize adsorption conditions such as methanol/water ratio, time, and pH. Sil@HIm-Im, which had the highest adsorption efficiency, was used in SPE as a sorbent. After 2.0 mL of loading samples, washing solvents were optimized as 6.0 mL and 4.0 mL of water for corn and peanut, 2.0 mL of acetonitrile, and 3.0 mL of methanol. 3.0 mL of methanol/acetic acid (2.0% vol.) was investigated as an elution solvent. Finally, 0.009 μg/g and 0.023 μg/g of aflatoxin B1 were obtained in corn and peanut extract with recoveries of 80.0%–103.3% and RSDs of 2.37%–6.58%.

Highlights

Aflatoxins (AFs) such as B1, B2, G1, and G2 are natural secondary metabolites that are produced by molds [1]
In the FT-IR spectra (Figure 3), silica showed a wide peak at 3429 cm−1 and this peak belonged to the –OH group
The peaks at 2917 cm−1 belonged to the carbon chain on the ionic liquids (ILs) group, the peak area increased with the carbon chain length increasing [33, 34]

Summary

Introduction

Aflatoxins (AFs) such as B1, B2, G1, and G2 are natural secondary metabolites that are produced by molds [1]. Large amount of solvents especially organic solvents were regulated as another kind of pollutants in the process. Ventura et al used an Oasis HLB SPE cartridge with HPLC/MS to detect four aflatoxins in a herb, 2.0 mL of methanol/water (30 : 70, vol%) was used as a washing solvent, and 4.0 mL of methanol/water (80 : 20, vol%) was used as an elution solvent [16]. Liu et al synthesized an aptamer-functionalized magnetic agarose microsphere and applied it in SPE to adsorb AFB1 and AFB2 with an Journal of Analytical Methods in Chemistry

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