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Abstract
Adipose tissue contains multipotent cells known as adipose-derived stem/stromal cells (ASCs), which have therapeutic potential for various diseases. Although the demand for adipose tissue for research use remains high, no adipose tissue bank exists. In this study, we attempted to isolate ASCs from cryopreserved adipose tissue with the aim of developing a banking system. ASCs were isolated from fresh and cryopreserved adipose tissue of rats and compared for proliferation (doubling time), differentiation capability (adipocytes), and cytokine (hepatocyte growth factor and vascular endothelial growth factor) secretion. Finally, ASCs (2.5 × 106) were intravenously infused into rats with spinal cord injury, after which hindlimb motor function was evaluated. Isolation and culture of ASCs from cryopreserved adipose tissue were possible, and their characteristics were not significantly different from those of fresh tissue. Transplantation of ASCs derived from cryopreserved tissue significantly promoted restoration of hindlimb movement function in injured model rats. These results indicate that cryopreservation of adipose tissue may be an option for clinical application.
Highlights
Regenerative medicine has advanced rapidly in recent years
We have reported that the transplantation of differentiated fat cells (DFAT) or adipose-derived stem/stromal cells (ASCs) improved hindlimb motor dysfunction in transplantation is being explored as a strategy promote tissue regeneration, variety ofeffect cells have rats with spinal cord injury (SCI) [12,13]
ASCs became apparently homogeneous during culture and passage, the isolated collagenase, the cells adhered on the culture dish and grew, similar to the treatment of fresh tissue
Summary
Regenerative medicine has advanced rapidly in recent years. Embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, and somatic stem cells have all become candidates for transplantation in regenerative therapy. ES or iPS cells have some limitations to their clinical application, including ethical concerns, problems with immunogenicity, and safety issues related to the potential for tumorigenicity [1]. Somatic stem cells, such as mesenchymal stem cells (MSCs), may offset these problems, as they are capable of self-renewal and can differentiate into multi-lineages [2,3,4]. MSCs have been found in many tissues, including adipose tissue, which possesses its own advantages for use in regenerative medicine [6,7,8] These advantages include its ubiquitous distribution, its accessibility, and the ease with which it can be harvested.
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