Isolation of Acinetobacter Spp. and its antimicrobial resistance pattern in all lower respiratory samples from ICU

Abstract

. are found in moist natural environment and hospital settings. In ICU . is the most common cause of Respiratory tract infection. . has evolved from a minor infection to one of the most virulent and multidrug-resistant, pathogens in intensive care units. Overuses of the antibiotics patients drug resistant pattern are increase and immune compromise patient are not recover early. This research will help in better infection control and a better knowledge of antibiotic resistance patterns in our area. The aim and objective of this study is isolation of . and its antimicrobial resistance pattern in all lower respiratory samples from ICU. All lower respiratory samples were collected (Sputum, BAL, ET etc.) samples was inoculated on MacConkey and blood agar. At 37 degrees Celsius, culture plates were incubated aerobically for 24 hours. Gram staining and biochemical test were used to identify the Acinetobacter species. All species was isolate further processed on the basis of AST by automated through vitek2 compact.Among 151 samples, 71 (47.01%) were culture positive. was isolated in 31 (43.66%). The number and percentage of in various clinical sample were sputum 14 (45.20%), ET 12 (38.70%), pleural fluid 3 (9.60%), BAL 2 (6.50%). The strains showed maximum resistance to Ampicillin (100%) and pipracillinetazobactum (94.0%), Ceftazidime (86%) followed by gentamycin (77%), Ciprofloxacin (72%). All the strains were sensitive to colistin and Polymyxin B (100%).The rise of resistant strains has resulted in fewer treatment choices. Because of the limited therapeutic options, infection prevention and control methods, including not only standard measures but also antibiotic management strategies in the ICU, are important.