Abstract
An enterotoxin was isolated from strainEscherichia coli 015 by salt precipitation and gel chromatography. In the process of isolation and purification the toxic activity of the preparation increased: by 60 times according to the ligated segment of rabbit intestine method and 66–100 times according to the skin test. The plateau and second fraction obtained by gel chromatography were inactive according to the ligated segment of intestine method but possessed permeability factor (PF) activity in the skin test. Two hypotheses were put forward: The vascular permeability factor and the diarrheagenic factor are possibly two different, substances (molecules) and the skin test is more sensitive as a method of determining toxicity than the ligated segment of rabbit intestine method.
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