Abstract
Six tetracycline resistance (TcR) gene transducing lambda phages were obtained from a lysate of Escherichia coli strain C600 (γb515b519) (R100–1). This strain was previously prepared by the following steps: cells were lysogenized with γb515b519 and multi-drug resistance factor R100–1 was introduced by mating. Each DNA extracted from six lambda phages called γptet, was characterized by the integration site of TcR gene in lambda whole genome. The site was determined by analysis of cleavage patterns of restriction endonuclease, EcoRI and HindIII and of heteroduplex figures under the electron microscope. The results indicated that three γptet phages, γptet 36, 41 and 105, accepted TcR gene at b2 region. The other three γptet phages, γptet 1, 27 and 115, accepted it at or near Q gene.
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