Abstract

This research provides insight into the expression of thermotolerance-related genes in Kluyveromyces marxianus by differential display polymerase chain reaction (DD-PCR) techniques. Fourteen differential expressed sequence tags (ESTs) were observed and one of them, SHWBY10 was confirmed to be positive by reverse Northern blot analysis. The sequence has the GenBank Accession ID No. CD374838. DNA sequencing showed that it encoded a 279 bp ORF containing 92 amino acids. Analysis of protein sequence indicated that it has significant sequence homology with a peroxisomal protein product (gi 50309315) from Kluyveromyces lactis. This discovery suggests this gene may be related to yeast thermotolerance.

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