Isolation of a floating cell strain from submerged cell cultures of Euphorbia marginata pursh

Abstract

A strain of floating cells, isolated from cell suspensions of Euphorbia marginata, is being maintained in standing flask culture without requirements of agitation or supplemental aeration for growth. The floating strain exhibits two-dimensional and three-dimensional growth patterns both of which are being maintained as sub-cultures. The strain consists of cells more uniform in morphology than the cells in suspension culture. Paths of cellular differentiation are similar in both cultures with resultant development of elongated and lignified cells. Isolated single cells or small cell groupings can be readily secured from both the floating cultures and the suspension cultures. Standing flask cultures as a technique can aid in the selection and maintenance of friable cell cultures on a liquid surface to supplement studies on agitated suspension cultures and agar base cultures.