Abstract
A dwarf plant isolated from anther culture of a normal genotype of birdsfoot trefoil (Lotus corniculatus L. cv. Leo) has been isolated, characterized, and maintained as an in vitro shoot-tip culture. The dwarf condition is the result of a much shorter internode length when compared with a normal genotype in shoot tip culture. Dwarf shoots which are typically rosette respond to increased GA3 levels by increased internode growth. Normal genotypes are inhibited and eventually die as a result of increased levels of GA3 in the culture medium. This dwarf has the normal tetraploid chromosome number of 24, and although stable in shoot tip cultures since 1977, has not survived outside of in vitro shoot tip cultures.
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