Isolation of a continuous epithelioid cell line, HBT-3, from a human breast carcinoma.

Abstract

SummaryA continuous line of human breast carcinoma cells, HBT-3, was established in culture following collagenase treatment of a mucus producing adenocarcinoma. The cells are epithelioid in appearance, multiply rapidly, have a cloning efficiency of approximately 70%, and exhibit an abnormal karyotype with a mode of 66-69 chromosomes/cell and 3 markers.Tumor material and pathological diagnosis were provided through the courtesy of Dr. J. D. Mashburn, Director of Laboratories, Washington Sanitarium and Hospital, Takoma Park, MD. Dr. C. S. Stulberg, Senior Research Associate, The Child Research Center of Michigan, Detroit, kindly performed the immunofluorescence cell typing tests.A portion of these studies was carried out at Bionetics Research Laboratories, Inc., Bethesda, MD, under contract 69-2160 from the Special Virus Cancer Program of the National Cancer Institute. Technical assistance of Cathy K. Smith, Nancy Tuttle Fuller, and Diane Robertson is gratefully acknowledged. The authors thank Drs. Peter J....