Abstract
The de novo nicotinamide adenine dinucleotide biosynthetic pathway ofEscherichia coli was investigated; using a cell-free extract of anadB mutant, we synthesized a precursor of quinolinic acid, a key intermediate in the pathway, from14C-aspartic acid. The synthesis of this compound was repressible by nicotinic acid. This compund was partially purified by ion-exchange column chromatography and then further purified and characterized by ascending partition chromatography on thin-layer plates. A metabolic scheme is presented which hypothesizes that the isolated precursor of quinolinic acid results from the amination of 2-butynedioic acid.
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