Abstract
RNA was extracted from the pancreatic islets of channel catfish in the presence of the ribonuclease inhibitor, diethyl pyrocarbonate (oxydiformate). High molecular weight RNA was observed on sucrose gradient analysis. enrichment of mRNA was achieved by oligo(2'-deoxythymidylic acid)-cellulose affinity chromatography. The mRNA fraction stimulated incorporation of [35S]methionine into protein up to 30-times the background in the wheat-germ cell-free system. Analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed two major proteins corresponding to molecular weights of 27 000 and 11 000. These proteins were not observed in the absence of mRNA or in the presence of mRNAs from other tissues. They were also synthesized in the ascites tumour cell-free system. No protein co-migrating with proinsulin or insulin was detected in either the ascites or wheat-germ cell-free systems. Pancreatic islet slices also synthesized the proteins of 27 000 and 11 000 molecular weight and smaller ones as well.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
